Antibodies from IMGENEX: NF-kB Pathway

Nuclear factor ?B (NF-?B) is a nuclear transcription factor that regulates expression of a large number of genes that are critical for the regulation of apoptosis, viral replication, tumorigenesis, inflammation, and various autoimmune diseases. Composed of homo- and heterodimers of five members of the Rel family including NF-?B1(p50), NF-?B2 (p52), RelA , RelB, and c-Rel (Rel), activation of NF-?B is thought to be part of a stress response as it is activated by a variety of stimuli including growth factors, cytokines, lymphokines, UV, pharmacological agents, and stress. In its inactive form, NF-?B is sequestered in the cytoplasm, bound by members of the I?B family of inhibitor proteins, which include I?Ba, I?Bß, IkB?, and IkBe. The various stimuli that activate NF-?B cause phosphorylation of I?B (through the I?B kinase complex consisting of at least three proteins: IKK1/a, IKK2/ß and IKK3/?), which is followed by its ubiquitination and subsequent degradation. This results in the exposure of the nuclear localization signals (NLS) on NF-?B subunits and the subsequent translocation of the molecule to the nucleus. In the nucleus, NF-?B binds with the consensus sequence (5’GGGACTTTCC-3’) of various genes, activating their transcription. The study of NF-?B activation has never been more important than today. As its role in diverse biological processes, such as arthritis, asthma, heart disease, chronic inflammation, cancer and certain neuro-degenerative disorders, become clear, it is becoming apparent that more tools using evolving technology are needed. IMGENEX offers a broad portfolio of different NF-?B detection tools and technologies for measurement, quantitation, and elucidation of critical pathway components. These tools include both phosphor and pan-specific mono- and polyclonal antibodies, novel nonradiometric readout assays for the quantification of I?Ba phosphorylation levels and NF-?B/p65, as well as peptide decoy inhibitors. Also available is our expanding line of validated plasmid-based and synthetic RNA interference kits targeting specific NF-?B related proteins.

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